Related Experiment Video
Updated: Aug 11, 2026
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
Published on: January 14, 2017
[Establishment of immortalized lymphoblastoid cell bank of a keloid pedigree]
Xin Yan1, Jian-hua Gao, Mei Song
1Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yenshin@etang.com
Objective:
To investigate a method for establishing immortalized lymphoblastoid cell bank of keloid pedigree so as to provide a long-term source of specimens for keloid research.
Methods:
With Epstein-Barr virus transformation, fresh and frozen blood samples collected from all members of the keloid pedigree were used respectively to establish the immortalized lymphoblastoid cell lines of B lymphocytes.
Results:
Twenty-seven immortalized lymphoblastoid cell lines of the keloid pedigree were obtained successfully, and all cell lines survived cryopreservation in liquid nitrogen.
Conclusions:
The immortalized lymphoblastoid cell bank of the keloid pedigree can preserve the whole gene information and provide long-term DNA sources for keloid research. Preparation of the cell lines with fresh blood is more efficient than that with frozen blood.
