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Updated: Aug 11, 2026
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
[Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity
Xiao-li Tu1, Hong-wei Liu, Cai-liang Li
1Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. renliwn@126.com
Objective:
To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.
Methods:
Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.
Result:
BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.
Conclusion:
BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
