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Related Experiment Videos

Haymaker gene expression in malignant and normal gynecologic tissues.

Mark E Borowsky1, Ballabh Das, Constantine A Axiotis

  • 1Department of Obstetrics and Gynecology, State University of New York Downstate Medical Center, Brooklyn, New York, USA.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|February 24, 2006
PubMed
Summary

Haymaker protein is highly expressed in normal and cancerous gynecologic epithelial cells, but not in connective tissue or smooth muscle cells. This suggests Haymaker is tissue-specific, not a marker for malignant transformation.

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Area of Science:

  • Oncology
  • Cell Biology
  • Gynecologic Pathology

Background:

  • Previous studies indicated high intracellular Haymaker protein levels in carcinoma and leukemia/lymphoma cell lines.
  • The role of Haymaker expression in neoplastic transformation versus tissue specificity remained unclear.

Purpose of the Study:

  • To investigate Haymaker protein expression in normal and cancerous gynecologic tissues.
  • To determine if Haymaker expression differentiates between neoplastic transformation and tissue specificity.

Main Methods:

  • Immunohistochemical staining and morphometric analysis of 85 gynecologic tissue specimens.
  • Utilized a specific polyclonal antibody against a Haymaker peptide for detection.

Main Results:

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  • Haymaker protein was highly expressed in normal uterine, vaginal, and cervical epithelial cells.
  • Poor or absent Haymaker expression was observed in connective tissue and smooth muscle cells.
  • No significant difference in Haymaker expression between malignant and normal gynecologic tissues was found.

Conclusions:

  • Haymaker protein expression is characteristic of gynecologic epithelial cells, both normal and malignant.
  • Haymaker protein expression is not a reliable marker for malignant transformation in gynecologic tissues.
  • Haymaker expression appears to be a marker for epithelial cell differentiation within gynecologic organs.