Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pneumococcal polysaccharides interact with human dendritic cells.

Ulrike Meltzer1, David Goldblatt

  • 1Immunobiology Unit, The Institute of Child Health, Great Ormond Street Hospital, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom.

Infection and Immunity
|February 24, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond survival: Multisystem long-term outcomes following HSCT in chronic granulomatous disease.

Journal of human immunity·2026
Same author

Determining the dose-response relationship for pneumococcal conjugate vaccines: a nested analysis of the fractional dose PCV trial.

Vaccine·2026
Same author

Immune transcriptomic differences in paediatric patients with SARS-CoV-2 compared to other lower respiratory tract infections.

bioRxiv : the preprint server for biology·2025
Same author

Enhanced adenoviral reactivity in Guillain-Barré syndrome after SARS-CoV-2 infection and vaccination.

Brain : a journal of neurology·2025
Same author

The development of functional opsonophagocytic assays to evaluate antibody responses to <i>Klebsiella pneumoniae</i> capsular antigens.

mSphere·2025
Same author

Clinical and regulatory development strategies for GBS vaccines intended for maternal immunisation in low- and middle-income countries.

Vaccine·2025
Same journal

<i>Pseudomonas aeruginosa</i> infection causes lysosomal dysfunction in the cystic fibrosis bronchial epithelium.

Infection and immunity·2026
Same journal

The role of probiotics in restoring and maintaining vaginal microbiome health: a review.

Infection and immunity·2026
Same journal

Pathological or preventative? Amyloid-β as an effector of innate immunity.

Infection and immunity·2026
Same journal

Effluxosomes and the evolution of metal resistance in <i>Mycobacterium tuberculosis</i>.

Infection and immunity·2026
Same journal

Characterization of an isogenic <i>bimA</i> mutant in the ATS2021 strain of <i>Burkholderia pseudomallei</i>.

Infection and immunity·2026
Same journal

HnRNPA2B1 tunes antimycobacterial immune responses in macrophages through alternative splicing of <i>Irgm1</i>.

Infection and immunity·2026
See all related articles

Human dendritic cells (DCs) internalize pneumococcal capsular polysaccharides (PPS). While PPS alone doesn't mature DCs, it alters their response to lipopolysaccharide (LPS), increasing IL-10 and decreasing IL-12 production.

Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Dendritic cells (DCs) are key antigen-presenting cells, crucial for initiating adaptive immune responses.
  • The role of DCs in response to polysaccharide antigens, particularly in humans, is not well understood.
  • Pneumococcal capsular polysaccharides (PPS) are critical virulence factors of Streptococcus pneumoniae.

Purpose of the Study:

  • To investigate the interaction between human monocyte-derived DCs and pneumococcal capsular polysaccharides (PPS) in vitro.
  • To determine if PPS influences DC maturation, cytokine production, and response to secondary stimuli.
  • To elucidate the mechanisms of PPS uptake by DCs.

Main Methods:

  • Human monocyte-derived DCs were generated and incubated with fluorescently labeled PPS.

Related Experiment Videos

  • DC uptake, maturation (phenotypic changes), and cytokine production (IL-12, IL-10) were assessed.
  • DCs were exposed to various PPS serotypes with or without Escherichia coli lipopolysaccharide (LPS) or tetanus toxoid.

    • Main Results:

    • Immature DCs efficiently took up PPS, localizing it to late endosomal compartments.
    • Uptake was dependent on cytoskeletal rearrangements and phosphatidylinositol 3-kinase (PI3K) signaling.
    • PPS alone did not induce DC maturation or significant cytokine production.
    • Co-exposure to PPS and LPS resulted in a significant shift in cytokine balance: increased IL-10 and decreased IL-12 production compared to LPS alone.
    • This modulatory effect was specific to PPS and not observed with tetanus toxoid.

    Conclusions:

    • Human DCs internalize pneumococcal capsular polysaccharides via mechanisms involving actin cytoskeleton and PI3K.
    • Pneumococcal capsular polysaccharides do not directly induce DC maturation but can modulate DC responses to inflammatory stimuli like LPS.
    • This modulation, characterized by an altered IL-10/IL-12 cytokine balance, may significantly impact DC-driven T-cell priming and subsequent immune responses to pneumococcal infections.