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Modulation of human neutrophil functions in vitro by Treponema denticola major outer sheath protein

Bina Puthengady Thomas1, Chun Xiang Sun, Elena Bajenova

  • 1CIHR Group in Matrix Dynamics and Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada M5S 3E2.

Infection and Immunity
|February 24, 2006
PubMed

Insights

Treponema denticola major outer sheath protein (Msp) impairs human immune cell function. Msp selectively inhibits polymorphonuclear leukocytes (PMNs) chemotaxis and phagocytosis by affecting their cytoskeleton.

Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Polymorphonuclear leukocytes (PMNs) are crucial immune cells.
  • Treponema denticola major outer sheath protein (Msp) is a key bacterial virulence factor.

Purpose of the Study:

  • To investigate the effect of Msp on human PMN functions.
  • To determine the specific mechanisms by which Msp impacts PMN activity.

Main Methods:

  • Human PMNs were pretreated with Msp.
  • Chemotaxis, phagocytosis, calcium signaling, and actin assembly were measured.
  • Oxidative responses and apoptosis were assessed.

Main Results:

  • Msp inhibited fMLP-induced chemotaxis and phagocytosis of IgG-coated microspheres.
  • Msp reduced fMLP-stimulated calcium transients and actin assembly.
  • Msp did not affect oxidative responses or induce apoptosis.

Conclusions:

  • Msp selectively impairs PMN chemotaxis and phagocytosis.
  • Msp affects the PMN cytoskeleton, leading to functional deficits.
  • Msp's impact on PMN function may contribute to T. denticola pathogenesis.

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