Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile QCM matrix system for online and high-throughput bio-sensing.

G Steve Huang1, Menq-Te Wang, Meng-Yen Hong

  • 1Institute of Nanotechnology, National Chiao Tung University, Hsinchu 300, Taiwan. gstevehuang@mail.nctu.edu.tw

The Analyst
|February 24, 2006
PubMed
Summary

This study introduces a novel quartz crystal microbalance (QCM) sensor matrix for parallel DNA detection. The expandable system demonstrates high sensitivity for labeled DNA samples, paving the way for advanced biosensing applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A protein transistor made of an antibody molecule and two gold nanoparticles.

Nature nanotechnology·2012
Same author

Dosage-dependent induction of behavioral decline in Caenorhabditis elegans by long-term treatment of static magnetic fields.

Journal of radiation research·2012
Same author

Self-assembly of an antireflective moth-like structure using antibody-functionalized nanowires and nanopore arrays.

Nanotechnology·2011
Same author

Topographic control of the growth and function of cardiomyoblast H9c2 cells using nanodot arrays.

Biomaterials·2011
Same author

Size-dependent impairment of cognition in mice caused by the injection of gold nanoparticles.

Nanotechnology·2010
Same author

A Nanodot Array Modulates Cell Adhesion and Induces an Apoptosis-Like Abnormality in NIH-3T3 Cells.

Nanoscale research letters·2010

Area of Science:

  • Biosensing and Nanotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Quartz crystal microbalance (QCM) is a sensitive mass-detection technique.
  • Parallel detection of multiple analytes is crucial for high-throughput screening.
  • Developing versatile sensor platforms for labeled DNA detection remains an active research area.

Purpose of the Study:

  • To develop and characterize a 3x3 QCM sensor matrix for parallel and comparative detection of labeled DNA samples.
  • To evaluate the sensitivity and specificity of the QCM matrix for biotin- and DIG-labeled DNA.
  • To demonstrate the expandability of the QCM matrix design for future applications.

Main Methods:

  • Fabrication of a 3x3 QCM sensor matrix on an A-T cut quartz crystal.

Related Experiment Videos

  • Utilized a single oscillator circuit with programmable time-shared electronic relays for sequential QCM activation.
  • Coated individual QCMs with streptavidin or anti-DIG antibodies for specific DNA capture.
  • Monitored detection using various concentrations of biotin- and DIG-labeled DNA samples.

    • Main Results:

    • The QCM sensor matrix achieved parallel and comparative online detection of labeled DNA samples.
    • Individual QCMs exhibited sensitivity in the pictogram regime.
    • Specific detection of biotin- and DIG-labeled DNA was confirmed using antibody-functionalized QCMs.
    • The sensor matrix demonstrated a fundamental resonating frequency of 40 MHz with gold electrodes of 0.8 mm diameter.

    Conclusions:

    • The developed 3x3 QCM sensor matrix is a viable platform for sensitive, parallel detection of labeled DNA.
    • The system's design is modular and expandable, offering potential for increased sensor density and versatility.
    • This technology holds promise for applications in diagnostics, molecular biology, and high-throughput screening.