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Related Experiment Videos

Generation of an Fgf9 conditional null allele.

Yongshun Lin1, Guoqin Liu, Fen Wang

  • 1Center for Cancer Biology and Nutrition, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030-3303, USA.

Genesis (New York, N.Y. : 2000)
|February 24, 2006
PubMed
Summary

Fibroblast growth factor 9 (FGF9) is crucial for development and adult tissue function. A new conditional null allele allows researchers to study FGF9's role in adult tissues without causing early death.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The fibroblast growth factor (FGF) family, comprising 22 regulatory polypeptides, influences numerous cellular processes.
  • FGF9 is vital for embryogenesis and maintaining adult tissue homeostasis.
  • Complete Fgf9 gene disruption causes neonatal lethality and lung malformations, limiting studies in adult tissues.

Purpose of the Study:

  • To create a tool for investigating FGF9's function in adult tissues.
  • To enable spatiotemporal and tissue-specific disruption of the Fgf9 gene.

Main Methods:

  • Generation of an Fgf9 conditional null allele using gene targeting in mouse embryonic stem cells.
  • Introduction of loxP sites flanking exon 1 of the Fgf9 gene.
  • Validation of the conditional null allele's function with Cre recombinase.

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Main Results:

  • The generated allele is a true conditional null, retaining wildtype activity.
  • Recombination mediated by Cre recombinase effectively converts the allele to a null state.
  • This tool circumvents neonatal lethality associated with complete Fgf9 disruption.

Conclusions:

  • The Fgf9 conditional null allele is a valuable research tool.
  • It facilitates the study of FGF9's roles in adult tissue homeostasis and function.
  • This advancement overcomes previous limitations in FGF9 research.