[Study on increasing anti-fungi activity of marine microbes by mixed culture]

The effect of mixed culture on the anti-fungi activity of marine microbes metabolites was evaluated. Based on optimization of monoculture, orthogonal design was used to approach the optimal region of the medium composition of mixed fermentation. The mixed culture showing anti-fungi activity of marine microbes was enriched obviously, compared with monoculture and fermentation mixture. The MIC (minimal inhibitory concentration) reduced from 156 microL/mL, 125 microL/mL by monoculture and 250 microL/mL, 218 microL/mL by mixture of monoculture fermentation to 32 microL/mL, 28 microL/mL, indicate 200% to 300% increase separately in fungi inhibition with mixed culture.