Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superoxide generation in leukocytes and vitamin E.

T Okano1, H Tamai, M Mino

  • 1Department of Pediatrics, Osaka Medical College, Takatsuki, Japan.

International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of Supine-Use Wearable Cyborg for Treatment of Neurogenic Bowel Dysfunction after Spinal Cord Injury.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

A nationwide survey of pediatric acquired demyelinating syndromes in Japan.

Neurology·2016
Same author

Complex molecular genetic abnormalities involving three or more genetic mutations are important prognostic factors for acute myeloid leukemia.

Leukemia·2015
Same author

Development of low-fluorescence thick photoresist for high-aspect-ratio microstructure in bio-application.

Biomicrofluidics·2015
Same author

Inhibition of S100A6 induces GVL effects in MLL/AF4-positive ALL in human PBMC-SCID mice.

Bone marrow transplantation·2014
Same author

Optimal administration time of intramuscular midazolam premedication.

Journal of anesthesia·2013

Vitamin E supplementation does not impair polymorphonuclear cell (PMN) function in humans or preterm infants. Oral vitamin E intake did not enhance PMN function, suggesting a safe dosage range for immune support.

Area of Science:

  • Immunology
  • Nutritional Science
  • Biochemistry

Background:

  • Polymorphonuclear cells (PMNs) are crucial immune cells involved in fighting infections.
  • Superoxide generation by PMNs is a key indicator of their functional activity.
  • Vitamin E is an antioxidant with potential immunomodulatory effects.

Purpose of the Study:

  • To investigate the effect of vitamin E on superoxide generation in PMNs.
  • To determine safe and effective dosage ranges of vitamin E for PMN function.
  • To assess vitamin E's impact on PMN function in human adults and premature infants.

Main Methods:

  • Chemiluminescence assay using Cypridina luciferin analogue to measure superoxide production in PMNs.
  • Exposure of PMN cell suspensions to varying concentrations of vitamin E.

Related Experiment Videos

  • Administration of vitamin E to rats, human adults, and premature infants, followed by PMN analysis.

    • Main Results:

    • High vitamin E concentrations (>80 µg/10^9 cells) suppressed PMN chemiluminescence.
    • Both very low (<2 µg/10^9 cells) and very high (>50 µg/10^9 cells) vitamin E levels inhibited superoxide production.
    • Oral vitamin E supplementation in adults and infants did not lead to PMN vitamin E levels exceeding 30 µg/10^9 cells, with no observed impairment or enhancement of function.

    Conclusions:

    • Oral vitamin E administration, even in premature infants, does not appear to impair PMN function.
    • Intramuscular administration in rats achieved potentially dangerous high vitamin E levels in PMNs.
    • Vitamin E supplementation within the tested oral ranges does not enhance PMN function in adults or infants.