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Related Experiment Videos

Compressive force induces osteoblast apoptosis via caspase-8.

Y Goga1, M Chiba, Y Shimizu

  • 1Division of Orthodontics and Dentofacial Orthopedics, Department of Oral Health and Development Sciences, School of Dentistry, Tohoku University, 4-1 Seiryo-cho, Aoba-ku, Sendai 980-8575, Japan. goga@mail.tains.tohoku.ac.jp

Journal of Dental Research
|February 25, 2006
PubMed
Summary

Excessive orthodontic force causes cell death. Compressive force induces apoptosis in human osteoblast-like cells via caspase-8 signaling, impacting periodontal remodeling during tooth movement.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Orthodontics

Background:

  • Periodontal remodeling is essential for orthodontic tooth movement.
  • Excessive orthodontic forces can lead to cell death in periodontal tissues.
  • The specific mechanisms of cell death induced by compressive forces are not fully understood.

Purpose of the Study:

  • To investigate whether continuous compressive force induces apoptosis in human osteoblast-like cells (MG-63 cells) in vitro.
  • To elucidate the signaling pathway involved in compressive force-induced apoptosis.

Main Methods:

  • Application of continuous compressive force to MG-63 cells in vitro.
  • Assessment of cell viability and morphology.
  • TUNEL assay to quantify apoptosis.

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  • Measurement of caspase-3 activity and evaluation of inhibitor effects (caspase-8 and caspase-9 inhibitors).

    • Main Results:

    • Compressive force caused irregular cell alignment and decreased cell viability.
    • TUNEL analysis revealed a time- and force-dependent increase in apoptotic cells.
    • Caspase-3 activity increased with compressive force magnitude.
    • Caspase-8 inhibition significantly reduced caspase-3 activity, while caspase-9 inhibition had no effect.

    Conclusions:

    • In vitro application of compressive force induces apoptosis in MG-63 cells.
    • Apoptosis is mediated through the activation of caspase-3 via the caspase-8 signaling cascade.
    • Findings provide insights into the cellular mechanisms underlying orthodontic force-induced tissue remodeling and potential cell death.