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[Storage technique on artificial seeds of Pinellia ternata]
Jian-ping Xue1, Ai-min Zhang, Wei Sheng
1Department of Biology, Huaibei Coal Industry Teachers, Colloge, China. xuejp2000@yahoo.com.cn
Objective:
To study the storage technique on artificial seeds of Pinellia ternata.
Method:
Microtubers were used as experiment materials. By using orthogonal experiment, the sodium alginate, added with GA3 badistan, chitosan and sodium benzoate, was used as seed vessel. The artificial seeds were stored respectively under 25 degrees C and 4 degrees C for 30 days, then, calculated the germination rate.
Result And Conclusion:
The sodium alginate(3%) added with GA3(0.1 mg x L(-1)), sodium benzoate(0.2%), badistan(1.0%), ClO2(0.1%) and chitosan(0.2%), were used as artificial seed vessel. Stored under (25 +/- 1) degrees C for 30 days, and the germination rate was over 65%. The sodium alginate(3%), added with GA3(0.1 mg x L(-1)), sodium benzoate(0.2%), badistan(1.0%), ClO2(0.2%) and chitosan(0.1%), were used as artificial seed vessel. Stored under 4 degrees C for 30 days, and the germination rate was over 80%.
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