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Published on: July 1, 2013
Molecular and biochemical analysis of ceruloplasmin expression in rabbit and rat ciliary body
Rubens Bertazolli-Filho1, Eduardo Miguel Laicine, Antonio Haddad
1Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brazil.
Purpose:
To verify the capability of rabbit and rat ciliary body to synthesize and secrete ceruloplasmin.
Methods:
Isolated ciliary body (CB) was cultured in the presence of [35S]-methionine, and the incubation medium was processed for immunoprecipitation. Total RNA from CB was processed for RT-PCR, and the amplification products were sequenced. Also, sections of CB were immunostained for the localization of ceruloplasmin.
Results:
A labeled peptide, having a molecular weight of about 135 kDa, the expected size of ceruloplasmin, was immunopurified in the incubation media from both animal species. The RT-PCR and sequencing experiments detected the presence of ceruloplasmin mRNA in rat samples. Both layers of rabbit and rat ciliary epithelium (CE) exhibited ceruloplasmin reactivity after immunohistochemical processing.
Conclusions:
Taken altogether, these results indicate the CB, particularly its epithelium, as one of the possible sources of the ocular intrinsic ceruloplasmin.
