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Chemical synthesis and expression of a gene coding for bovine liver phosphotyrosine-protein phosphatase
G Raugei1, R Marzocchini, A Modesti
1Department of Biochemical Sciences, University of Florence, Italy.
Biochemistry International
|January 1, 1991
Abstract:
A chemically synthesized polynucleotide sequence coding for bovine liver low molecular weight acid phosphatase (which possesses phosphotyrosine-protein phosphatase activity) has been cloned in a E. coli expression vector. The recombinant protein retains correct affinity for substrate and inhibitors but shows a reduced specific activity.