Related Experiment Video
Updated: Aug 11, 2026

Optimized Method for Cultivation and Microbial Bioaugmentation of Typha latifolia (Cattail)
Published on: July 25, 2025
[Explant culture of Trifolium pratense L]
M Kasparová1, T Siatka, J Spilková
1Farmaceutická fakulta Univerzity Karlovy, Hradec Králové, Katedra farmakognozie. kasparo@faf.cuni.cz
Abstract:
The red cloverl seems to be a prospective source of flavonoids and isoflavonoids with phytoestrogenic activity. Young seedlings of four different varieties of Trifolium pratense L. were employed to derive callus and suspension cultures. The optimal medium for cultivation is Gamborg's cultivating medium with an addition of 2 mg x l(-1) of 2,4-dichlorphenoxyacetic acid and 2 mg x l(-1) of 6-benzylaminopurine. On the basis of growth and production characteristics, subcultivating intervals of 29 to 43 days in callus culture and 16 to 23 days in suspension culture were determined. TLC and HPLC revealed that the explant culture of Trifolium pratense L. contains the isoflavonoid formononetin.
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