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Updated: Aug 11, 2026
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017
[Detection of CD95 and CD95L mRNA expression after liver transplantation using SYBR real-time PCR during acute
Li-xin Yu1, Shu-ling Han, Yun Miao
1Center of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To detect the expression of CD95 and CD95L mRNAs after liver transplantation and investigate the role of CD95 and CD95L in acute liver allograft rejection.
Methods:
The expressions of CD95 and CD95L mRNAs of peripheral blood lymphocyte from 56 liver allograft recipients were examined using SYBR real-time PCR.
Results:
CD95 and CD95L mRNA levels in the recipients with acute rejection were significantly higher than those without rejection (P<0.01), and the elevation occurred about 2 days earlier than that of alanine aminotransferase and aspartate aminotransferase.
Conclusion:
CD95 and CD95L are related to acute liver allograft rejection and their mRNA expression level may serve as an indicator for prediction and diagnosis of acute rejection episodes.
