Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA extraction using a tetramethyl orthosilicate-grafted photopolymerized monolithic solid phase.

Jian Wen1, Christelle Guillo, Jerome P Ferrance

  • 1Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22904, USA.

Analytical Chemistry
|March 1, 2006
PubMed
Summary

A new silica-based monolithic column offers high-capacity, high-efficiency DNA extraction. This method optimizes binding and elution for microscale devices, outperforming commercial kits for whole blood samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Basal metabolic rate of adults aged 40-60 years using indirect calorimetry].

Wei sheng yan jiu = Journal of hygiene research·2026
Same author

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same author

Correlations between endocrine-metabolic characteristics and body fat distribution, appetite, growth, and memory in children with Prader-Willi syndrome.

Clinics (Sao Paulo, Brazil)·2026
Same author

The Disproportionate Burden of Cardiovascular Disease in Mild-to-Moderate COPD.

International journal of chronic obstructive pulmonary disease·2026
Same author

Heterogeneity of cancer-associated fibroblast subtypes in the prostate cancer microenvironment and their clinical implications: prognostic model construction and therapeutic target exploration based on bioinformatics analysis.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2026
Same author

An explainable machine learning method for identifying key atherogenic lipid biomarkers in abdominal obesity among the Southern Chinese population.

Lipids in health and disease·2026

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Solid-phase extraction is crucial for isolating DNA.
  • Existing methods often face limitations in capacity, efficiency, or scalability.
  • Microscale devices require efficient and high-capacity extraction matrices.

Purpose of the Study:

  • To develop a novel, high-capacity, high-efficiency DNA extraction method.
  • To optimize a photopolymerized silica-based monolithic column for DNA binding and elution.
  • To evaluate the performance of the developed method for microscale DNA extraction.

Main Methods:

  • Fabrication of silica-based monolithic columns in fused-silica capillaries using sol-gel chemistry.
  • Optimization of monomer composition (3-(trimethoxysilyl)propyl methacrylate - TMSPM) and fabrication conditions.

Related Experiment Videos

  • Surface modification via grafting tetramethyl orthosilicate (TMOS) to enhance DNA binding capacity.
  • Investigation of extraction efficiency and capacity using prepurified human genomic DNA and whole blood samples.

    • Main Results:

    • Optimized TMSPM monoliths demonstrated high DNA binding capacity and stability.
    • TMOS surface modification significantly increased DNA binding capacity, with 85% v/v TMOS found optimal.
    • Reduced hydrolysis time improved monolith robustness and enabled higher binding capacity.
    • Minimal elution buffer volume (1 μL) achieved DNA concentration.
    • Shortened (2 cm) monolithic columns achieved >85% extraction efficiency for 100 ng DNA.
    • Submicroliter whole blood extraction showed higher efficiency than a commercial kit.

    Conclusions:

    • The novel silica-based monolithic column represents an attractive alternative for DNA solid-phase extraction.
    • The method offers high capacity, high efficiency, and a DNA concentrating effect suitable for microscale applications.
    • This technology has potential for extracting other biologically active molecules in microdevices.