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Updated: Aug 11, 2026
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Published on: August 5, 2008
Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue
Andrew B Goulter1, Daniel W Harmer, Kenneth L Clark
1Pharmagene Laboratories Ltd, Royston, Hertfordshire, SG8 5HD, UK. andrew.goulter@pharmagene.com
Background:
Low density arrays (LDAs) have recently been introduced as a novel approach to gene expression profiling. Based on real time quantitative RT-PCR (QRT-PCR), these arrays enable a more focused and sensitive approach to the study of gene expression than gene chips, while offering higher throughput than more established approaches to QRT-PCR. We have now evaluated LDAs as a means of determining the expression of multiple genes simultaneously in human tissues and cells.
Results:
Comparisons between LDAs reveal low variability, with correlation coefficients close to 1. By performing 2-fold and 10-fold serial dilutions of cDNA samples in the LDAs we determined a clear linear relationship between the gene expression data points over 5 orders of magnitude. We also showed that it is possible to use LDAs to accurately and quantitatively detect 2-fold changes in target copy number as well as measuring genes that are expressed with low and high copy numbers in the range of 1 x 10(2)-1 x 10(6) copies. Furthermore, the data generated by the LDA from a cell based pharmacological study were comparable to data generated by conventional QRT-PCR.
Conclusion:
LDAs represent a valuable new approach for sensitive and quantitative gene expression profiling.
