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Updated: Jul 2, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Effect of sample solvent on the chromatographic peak shape of analytes eluted under reversed-phase liquid
Sonia Keunchkarian1, Mario Reta, Lilian Romero
1División Química Analítica y CIDEPINT, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Abstract:
Peak-shape problems represent the most common troubles in liquid chromatography. Distorted peaks are causes for integration problems, and very often for poor resolutions. Sample and standard solvent different from that of the eluent is one of the possible reasons for anomalous peak-shapes. The injection of a pulse of different viscosity from that of the mobile phase is the underlying cause of distortions in early eluting bands. Strong eluting solvents (of exactly equal viscosity coefficients) are cause of band broadening, not of peak distortions. The goal of the present work is to critically evaluate the significant role of sample solvent upon the chromatographic band distortion. Practical solutions for situations where chromatographic analysis is performed under conditions far from ideal as a result of time constrains related to sample preparation are also discussed.
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