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Related Experiment Videos

Ca2+ -induced Ca2+ release through localized Ca2+ uncaging in smooth muscle.

Guangju Ji1, Morris Feldman, Robert Doran

  • 1Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA.

The Journal of General Physiology
|March 1, 2006
PubMed
Summary

Calcium-induced calcium release (CICR) in smooth muscle involves localized calcium release. This study shows CICR is not spatially restricted and can involve both ryanodine receptors and inositol trisphosphate receptors.

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Area of Science:

  • Cellular Biology
  • Physiology

Background:

  • Calcium-induced calcium release (CICR) is crucial for smooth muscle function, occurring as spontaneous sparks or triggered release.
  • CICR exhibits spatial localization, with release concentrated in specific subcellular regions.

Purpose of the Study:

  • To investigate how localized calcium increases activate sarcoplasmic reticulum (SR) release in urinary bladder smooth muscle cells.
  • To elucidate the molecular and biophysical mechanisms of CICR using two-photon flash photolysis (TPFP).

Main Methods:

  • Utilized two-photon flash photolysis (TPFP) of caged calcium (DMNP-EDTA) in Fluo-4 loaded smooth muscle cells.
  • Analyzed calcium release patterns, including sparks and waves, in response to localized calcium uncaging.

Main Results:

Related Experiment Videos

  • TPFP triggered localized calcium release (sparks, waves) demonstrating release follows a localized calcium rise.
  • CICR activation was not confined to specific regions, occurring throughout the cell near photolysis sites.
  • Ryanodine receptors (RYRs) dominated CICR, but a secondary, high-threshold release sensitive to xestospongin C (InsP3R antagonist) was observed.

Conclusions:

  • Localized calcium release activates CICR, similar to sarcolemmal calcium channel activation, with a strong dependence on type 2 RYRs.
  • CICR release is not spatially restricted to a few subcellular regions.
  • Inositol trisphosphate receptors (InsP3Rs) can mediate calcium release at high local calcium concentrations.