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Assembling an intermediate filament network by dynamic cotranslation.

Lynne Chang1, Yaron Shav-Tal, Tatjana Trcek

  • 1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. l-chang1@northwestern.edu

The Journal of Cell Biology
|March 1, 2006
PubMed
Summary
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Researchers observed messenger RNA (mRNA) and protein interactions in vivo. Messenger ribonucleoproteins (mRNPs) move along microtubules, initiating translation upon stopping, forming "translation factories" for protein synthesis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeletal Dynamics

Background:

  • Intermediate filaments (IFs) form essential cytoskeletal networks.
  • Peripherin is a key component of neuronal intermediate filaments.
  • Understanding the regulation of peripherin synthesis and assembly is crucial for neuronal function.

Purpose of the Study:

  • To investigate the in vivo dynamics of peripherin messenger RNA (mRNA) and its protein product.
  • To elucidate the mechanisms governing mRNA transport, translation, and protein assembly.
  • To define the relationship between mRNA dynamics and cytoskeletal organization.

Main Methods:

  • Studied the synthesis and assembly of peripherin intermediate filaments (IFs) in vivo.
  • Tracked the movement and localization of peripherin mRNA-containing particles (messenger ribonucleoproteins [mRNPs]).

Related Experiment Videos

  • Analyzed the translational status and assembly of peripherin protein.

    • Main Results:

    • Peripherin mRNPs predominantly move along microtubules (MT).
    • mRNPs are translationally silent during movement, initiating translation upon cessation of motion.
    • Multiple mRNAs can cluster in mRNPs, forming "translation factories" for amplified protein synthesis.
    • Peripherin undergoes cotranslational assembly into precursor particles for IFs.
    • mRNA clustering depends on MT, RNA regulatory sequences, and the nascent protein.

    Conclusions:

    • Dynamic cotranslation links the motility and targeting of peripherin mRNPs, translational control, and IF cytoskeletal assembly.
    • This process allows for localized and amplified protein synthesis, contributing to cytoskeletal organization.
    • The findings provide new insights into the regulation of protein synthesis and cytoskeletal dynamics in vivo.