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Updated: Aug 11, 2026

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
Published on: September 8, 2011
Muscarinic acetylcholine receptor subtypes in human corneal epithelium and endothelium
Matthias Grueb1, Eva Reinthal, Jens Martin Rohrbach
1Department of Ophthalmology, University of Tuebingen, Schleichstr 12, D-72076, Tuebingen, Germany. matthias.grueb@med.uni-tuebingen.de
Background:
Muscarinic acetylcholine receptors are located throughout the body. The demonstration of muscarinic receptors in corneal tissue has been inconsistent. Using freshly fixed human corneal tissue, we show a complete profile of muscarinic receptor subtypes in human corneal epithelium and endothelium.
Methods:
Muscarinic receptor sites were studied using immunocytochemistry, immunofluorescence and immunoblotting.
Results:
Antibodies to M2, M4 and M5 muscarinic receptor subtypes bound in human corneal epithelium and endothelium. No binding was found for antibodies to M1 and M3 muscarinic receptor subtypes.
Conclusions:
Our studies indicate the presence of M2, M4 and M5 muscarinic acetylcholine receptor subtypes in human corneal epithelium and endothelium. These receptors may play a role in the regulation of corneal homeostasis, other functions, like wound healing, or the pathogenesis of corneal diseases.
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