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Updated: Aug 11, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
The biosynthetic gene cluster for the beta-lactam antibiotic tabtoxin in Pseudomonas syringae
Thomas G Kinscherf1, David K Willis
1USDA/ARS Vegetable Crops Research Unit and Department of Plant Pathology, University of Wisconsin - Madison, Madison, WI 53706, USA.
Abstract:
DNA sequence analysis revealed that the biosynthetic genes of the unusual beta-lactam antibiotic tabtoxin reside at the att site adjacent to the lysC tRNA gene in Pseudomonas syringae BR2. ORFs encoded within the region included ones with similarity to beta-lactam synthase and clavaminic acid synthase, as well as amino acid synthesis enzymes. Novel ORFs were present in a portion of the biosynthetic region associated with a toxin hypersensitivity phenotype. Tabtoxin resistance was associated with a fragment containing a major facilitator superfamily (MFS) transporter gene.
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