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Nucleotide sequence of IS402 from Pseudomonas cepacia
1Department of Microbiology, University of Massachusetts, Amherst 01003.
Gene
|June 15, 1991
Summary
IS402, a novel gene-activating element in Pseudomonas cepacia, was sequenced. This bacterial insertion sequence is unrelated to known elements, offering new insights into gene regulation.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- IS402 is a transposable element identified by its ability to enhance gene expression.
- Its role in modulating gene expression in Pseudomonas cepacia requires further characterization.
Purpose of the Study:
- To determine the nucleotide sequence of the IS402 element.
- To analyze the structural features of IS402.
- To compare IS402 with known bacterial insertion sequence elements.
Main Methods:
- Cloning of IS402 from the plasmid pTGL52 into the pBluescript KS+ vector.
- Determination of the nucleotide sequence of the 914-bp IS402 element.
- Bioinformatic analysis comparing the IS402 sequence against GenBank and EMBL databases.
Main Results:
- The nucleotide sequence of IS402 (914 bp) was determined.
- IS402 possesses terminal inverted repeats (17 bp) with one mismatch.
- Insertion of IS402 into Tn1 resulted in a 3-bp direct target duplication.
- Sequence comparison revealed IS402 is distinct from all previously identified bacterial insertion sequences.
Conclusions:
- IS402 represents a novel class of bacterial insertion sequence.
- Its unique structure and lack of homology suggest a distinct evolutionary origin.
- Further research is warranted to elucidate the precise mechanisms of gene activation by IS402.