Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microfluidic chambers for cell migration and neuroscience research.

Anne M Taylor1, Seog Woo Rhee, Noo Li Jeon

  • 1Department of Biomedical Engineering, University of California-Irvine, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2006
PubMed
Summary

Researchers developed versatile microfluidic chambers for cell migration and neuroscience. These devices, fabricated using soft lithography, enable precise control over cell environments for advanced biological research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Throughput Digital Decoding of Vascular Heterogeneity in Patient-Specific Tumor Microenvironments.

Advanced healthcare materials·2026
Same author

Aquaporin-1 sustains lymphangiogenic responses in hyperosmotic inflammatory microenvironments.

The Journal of experimental medicine·2026
Same author

A Microfluidic Cell Culture Platform for Modeling Aligned Peripheral Nerve Bundle, Connection, and Myelination.

Advanced healthcare materials·2026
Same author

Correction: Evaluating migration and cytotoxicity of tissue-resident and conventional NK cells in a 3D microphysiological system using live-cell imaging.

Lab on a chip·2025
Same author

Engineering a Whitlockite-Containing Macroporous Composite Cryogel with Silica Hybrid for Enhanced Vascularized Bone Regeneration.

ACS applied materials & interfaces·2025
Same author

Cytotoxic Chemotherapy in a 3D Microfluidic Device Induces Dendritic Cell Recruitment and Trogocytosis of Cancer Cells.

Cancer immunology research·2025

Area of Science:

  • Biotechnology
  • Cell Biology
  • Neuroscience

Background:

  • Microfluidic devices offer advanced capabilities for cell-based research.
  • Soft lithography provides a reproducible and accessible method for fabricating microfluidic chambers.
  • Existing methods may lack the precision or isolation required for complex cell studies.

Purpose of the Study:

  • To describe the fabrication and application of novel microfluidic chambers.
  • To present two distinct microfluidic chamber designs: one for chemotaxis and one for neuronal culture.
  • To highlight the advantages of soft lithography for creating these research tools.

Main Methods:

  • Fabrication of microfluidic chambers using soft lithography and replica molding.
  • Design and implementation of a microfluidic chemotaxis chamber for gradient generation.

Related Experiment Videos

  • Development of a multicompartment culture chamber for isolating neuronal cell bodies and processes.

    • Main Results:

    • Demonstrated the ability to generate precise and stable chemoattractant gradients.
    • Successfully created a chamber that fluidically isolates neuronal processes from cell bodies.
    • Confirmed compatibility of both devices with standard microscopy techniques (phase, DIC, fluorescence).

    Conclusions:

    • Soft lithography is a robust and accessible method for producing advanced microfluidic research tools.
    • The described microfluidic chambers facilitate detailed studies of cell migration and neuronal development.
    • These devices offer significant advantages for cell migration and neuroscience research in standard laboratory settings.