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Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing
P Manish Chandra1, James A Brannigan, Asmita Prabhune
1Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India.
Abstract:
The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
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