Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reciprocal homologous junctions generated in mouse cells.

L Desautels1, S Brouillette, P Chartrand

  • 1Canadian Red Cross Society, Blood Services, Montreal, Quebec.

Molecular & General Genetics : MGG
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Accurate determination of the Gibbs energy of Cu-Zr melts using the thermodynamic integration method in Monte Carlo simulations.

The Journal of chemical physics·2011
Same author

Illegitimate DNA integration in mammalian cells.

Gene therapy·2003
Same author

RNP localization and transport in yeast.

Annual review of cell and developmental biology·2001
Same author

An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast.

The Journal of cell biology·2001
Same author

The odyssey of a regulated transcript.

RNA (New York, N.Y.)·2001
Same author

She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA.

The EMBO journal·2000

Homologous recombination in mouse cells produces reciprocal junctions with a middle segment from a single parent, suggesting a gap repair mechanism. Double-strand breaks did not increase reciprocal junction formation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Homologous recombination is a fundamental process for DNA repair and genetic diversity.
  • Understanding the mechanisms of homologous recombination is crucial for fields ranging from cancer research to evolutionary biology.

Purpose of the Study:

  • To analyze reciprocal homologous junctions from intermolecular conservative homologous recombination in mouse cells.
  • To compare the contribution of parental sequences to reciprocal recombination products.
  • To investigate the role of double-strand breaks in homologous recombination.

Main Methods:

  • Analysis of reciprocal homologous junctions in mouse cells using an assay that does not require selectable gene reconstitution.
  • Introduction of multiple markers into parental homologous sequences.

Related Experiment Videos

  • Comparison of reciprocal junction segments to determine parental origin.

    • Main Results:

    • All analyzed recombinants exhibited a middle segment of uniparental origin in reciprocal junctions.
    • This uniparental segment occurred randomly within the homology region and could span over a thousand base pairs.
    • Introducing double-strand breaks in the homology region decreased, rather than enhanced, the proportion of reciprocal homologous junctions.

    Conclusions:

    • The findings support a gap repair model for homologous recombination in mouse cells, similar to that observed in yeast.
    • The random occurrence and size of uniparental segments provide insights into the dynamics of DNA repair during recombination.
    • Double-strand breaks may not be the primary drivers for reciprocal homologous junction formation in this context.