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Related Experiment Videos

Screening antibody-antigen interactions in parallel using Biacore A100.

Pär Säfsten1, Scott L Klakamp, Andrew W Drake

  • 1Biacore AB, SE-75450 Uppsala, Sweden.

Analytical Biochemistry
|March 3, 2006
PubMed
Summary

High-throughput label-free optical biosensor technology using the Biacore A100 system efficiently analyzes crude hybridoma samples. This method provides high-quality binding data, improving early selection of therapeutic monoclonal antibodies.

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Area of Science:

  • Biotechnology
  • Immunology
  • Analytical Chemistry

Background:

  • Label-free optical biosensor technology is crucial for characterizing monoclonal antibodies.
  • Early access to high-quality binding data enhances antibody selection for development.
  • Increased throughput is needed for efficient analysis of numerous samples.

Purpose of the Study:

  • To evaluate the Biacore A100 system for high-throughput analysis of monoclonal antibodies.
  • To demonstrate the system's capability in providing high-quality kinetic and binding data.
  • To assess the impact of parallel processing on protein interaction analysis throughput.

Main Methods:

  • Utilized the Biacore A100 protein interaction array system for automated analysis.
  • Analyzed 386 crude hybridoma samples in a 12-hour automated run.

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  • Performed higher resolution analysis and studied binding interactions under various buffer conditions.

    • Main Results:

    • The Biacore A100 system successfully identified hybridoma samples with desired kinetic profiles.
    • High-quality binding data was obtained with significantly increased throughput.
    • Parallel processing expanded analysis capacity while maintaining data integrity.

    Conclusions:

    • The Biacore A100 system offers a robust solution for high-throughput characterization of monoclonal antibodies.
    • This technology improves efficiency in selecting promising antibody candidates.
    • The system maintains high data quality, facilitating reliable antibody development.