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V-src genes in two cell lines of so-called RSV-transformed human cells are defective and inactive

H Ogura1, N Momozaki, T Fujiwara

  • 1Department of Virology, Cancer Institute, Okayama University Medical School, Japan.

Acta Medica Okayama
|April 1, 1991
PubMed

Insights

Rous sarcoma virus (RSV) could not be induced from human cells (KC and RSb) due to defective v-src genes. In contrast, RSV was inducible from transformed mouse and rat cells with complete v-src genes.

Area of Science:

  • * Molecular biology
  • * Virology
  • * Oncology

Background:

  • * Rous sarcoma virus (RSV) is a retrovirus known for its ability to transform cells.
  • * The v-src gene is crucial for RSV's transforming activity.
  • * Understanding the mechanisms of viral transformation and oncogene expression is vital in cancer research.

Purpose of the Study:

  • * To investigate the inducibility of RSV from human cell lines (KC and RSb).
  • * To determine the integrity and expression status of the v-src gene in these cells.
  • * To compare RSV inducibility and v-src gene status in human cells versus rodent cells.

Main Methods:

  • * Cell fusion assays with chick embryo cells (CEC).
  • * Wing web tests for tumor induction.
  • * DNA transfection experiments.
  • * Southern and Northern blot hybridization using a v-src probe.

Main Results:

  • * RSV induction was unsuccessful from KC and RSb human cells via cell fusion and DNA transfection.
  • * Analysis revealed defective v-src genes in KC and RSb cells, with no detectable expression.
  • * RSV-transformed mouse and rat cells possessed complete v-src genes and allowed for virus induction.

Conclusions:

  • * The inability to induce RSV from KC and RSb cells is attributed to defective and unexpressed v-src genes.
  • * The integrity of the v-src gene is essential for RSV transformation and inducibility.
  • * Human cell lines KC and RSb are not suitable for RSV rescue due to v-src gene defects.

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