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Inhibitory synaptic transmission governs inspiratory motoneuron synchronization.
Joy Y Sebe1, Johannes F van Brederode, Albert J Berger
1Graduate Program in Neurobiology and Behaviour, School of Medicine, University of Washington, Seattle, WA 98195-7290, USA. sebe@u.washington.edu
Journal of Neurophysiology
|March 3, 2006
Summary
Synchronous oscillations in the respiratory network increase with development. GABA and glycine neurotransmission are crucial for synchronizing neural activity and setting oscillation frequency in developing mice.
Area of Science:
- Neuroscience
- Developmental Biology
- Respiratory Physiology
Background:
- Neurons in the respiratory network generate synchronized action potentials called synchronous oscillations during breathing.
- The roles of inhibitory neurotransmitters in modulating these oscillations and respiratory rhythm during development are not fully understood.
Purpose of the Study:
- To investigate the developmental changes in synchronous oscillations within the respiratory network.
- To determine how GABAergic and glycinergic neurotransmission influence these oscillations and respiratory rhythm.
Main Methods:
- In vitro recordings of inspiratory activity from mouse medullary slices (P0-P11).
- Pharmacological manipulation using GABA(A) and Glycine receptor antagonists, Substance P, and zolpidem.
- Analysis of oscillation frequency, power, and peak integrated activity.
Main Results:
- Average oscillation frequency significantly increased from 17 Hz in neonates (P0-P6) to 38 Hz in juveniles (P7-P11).
- GABA(A) and Glycine receptor antagonists reduced oscillation power and increased peak activity in both age groups.
- Prolonging GABAergic synaptic current duration with zolpidem decreased oscillation frequency.
Conclusions:
- Respiratory network oscillation frequency increases during postnatal development.
- Both GABAergic and glycinergic neurotransmission are essential for synchronizing neural activity and contribute to respiratory rhythm.
- The kinetics of GABAergic synaptic currents play a critical role in determining oscillation frequency.