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Updated: Aug 11, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Oligonucleotides with cyclohexene-nucleoside building blocks: crystallization and preliminary X-ray studies of a
Koen Robeyns1, Piet Herdewijn, Luc Van Meervelt
1Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
Abstract:
Cyclohexene nucleic acids contain a cyclohexene ring instead of the normal beta-D-2'-deoxyribose. The cyclohexene oligonucleotide GTGTACAC was synthesized using phosphoramidite chemistry and standard protecting groups. Crystals of GTGTACAC were obtained at 289 K by the hanging-drop vapour-diffusion technique. The crystals diffract to 1.7 A resolution and belong to the trigonal space group R3, with unit-cell parameters a = 41.434, c = 66.735 A.
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