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Updated: Aug 11, 2026
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Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
Published on: January 6, 2017
Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans
Catherine Tricot1, Sebastien van Aelst, Ruddy Wattiez
1Institut de Recherches Microbiologiques JM Wiame, Belgium.
Abstract:
CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation.
