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Updated: Aug 11, 2026
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia
Frédéric Huché1, Philippe Delepelaire, Cécile Wandersman
1Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany. huche@pasteur.fr
Abstract:
Serratia marcescens is able to acquire iron using its haem-acquisition system (;has'), which contains an outer membrane receptor HasR and a soluble haemophore HasA. After secretion, HasA binds free haem in the extracellular medium or extracts it from haemoproteins and delivers it to the receptor. Here, the crystallization of a HasA-HasR complex is reported. HasA and HasR have been overexpressed in Escherichia coli and the complex formed and crystallized. Small platelets and bunches of needles of dimensions 0.01 x 0.1 x 1 mm were obtained. A native data set has been collected to 6.8 A.
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