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Related Experiment Videos

Cell handling using microstructured membranes.

Daniel Irimia1, Mehmet Toner

  • 1BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Shriners Hospital for Children and Harvard Medical School, Boston, MA 02114, USA.

Lab on a Chip
|March 3, 2006
PubMed
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This study introduces a novel microfluidic membrane for precise cell handling in lab-on-a-chip devices. It enables on-chip sample preparation, including blood sampling and cell concentration, enhancing scientific research and diagnostics.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Precise handling of cell suspensions is crucial for research and diagnostics.
  • Existing microfluidic techniques often require off-chip sample preparation.

Purpose of the Study:

  • To present a microfluidic design concept using microstructured membranes for on-chip sample preparation.
  • To enable common sample preparation procedures for eukaryotic cell suspensions in lab-on-a-chip devices.

Main Methods:

  • Development of a microstructured membrane for pneumatic actuation.
  • Implementation of metered sampling, cell concentration, and sequential reagent exposure.
  • Utilizing reversible decoupling of fluid flow and cell displacement.

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Main Results:

  • Demonstrated metered sampling of nanoliter blood volumes.
  • Achieved cell concentration increases up to three orders of magnitude.
  • Enabled uniform, sequential exposure of cells to reagents on-chip.

Conclusions:

  • The microstructured membrane offers a versatile platform for integrated sample preparation in microfluidic devices.
  • This design facilitates complex sequential procedures with minimal control steps.
  • Enhances capabilities for cell analysis in scientific research and clinical diagnostics.