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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

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Cell cultures from marine mammals

J T Cecil1, R F Nigrelli

  • 1Osborn Laboratories of Marine Sciences, Brooklyn, New York 11224, USA.

Journal of Wildlife Diseases
|October 1, 1970
PubMed

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media