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Updated: Aug 7, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
A kinetic spectrophotometric method for the determination of nitrite by stopped-flow technique
Rui-Yong Wang1, Xiang Gao, Ying-Tang Lu
1Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Abstract:
A novel and highly sensitive stopped-flow kinetic spectrophotometric method for the determination of nitrite, based on monitoring the variation in the absorbance of the intermediate within a very short period, has been developed. The optimum conditions for various parameters on which the reaction of nitrite with perphenazine depends, were investigated. It was found that the initial reaction rate increased linearly with increasing nitrite concentration in the range from 1.0 x 10(-8) to 6.0 x 10(-6) M. The detection limit was calculated to be 4.8 x 10(-9) M. This method was used for the determination of nitrite in natural and drinking-water with satisfactory results. The influence of cationic, non-ionic and anion surfactants was also studied in this work.
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