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[3H]hemicholinium-3 binding in rats with status epilepticus induced by lithium chloride and pilocarpine
K Yamada1, M D Saltarelli, J T Coyle
1Department of Psychiatry, Neuroscience, Pharmacology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Abstract:
The specific binding of [3H]hemicholinium-3 ([3H]HCh-3) and high-affinity [3H]choline uptake were measured in rats with status epilepticus induced by lithium and pilocarpine. The specific binding of [3H]HCh-3 in cortex and hippocampus from rats with status epilepticus increased to 2- to 3-fold of control while the striatal [3H]HCh-3 binding increased minimally. Scatchard analyses revealed that the observed changes resulted from an increase in Bmax of [3H]HCh-3 binding. High-affinity [3H]choline uptake remained unchanged. These results further implicate phospholipase A2 in the regulation of [3H]HCh-3 binding sites.