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Updated: Aug 3, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 9, 2014
Direct extraction of the mean particle size from a digital hologram
Loïc Denis1, Corinne Fournier, Thierry Fournel
1Laboratoire Traitement du Signal et Instrumentation, Unité Mixte de Recherche-Centre National de la Recherche Scientifique 5516, Bâtiment F, 10 Rue Barrouin, 42000 Saint-Etienne, France. loic.denis@univ-st-etienne.fr
Abstract:
Digital holography, which consists of both acquiring the hologram image in a digital camera and numerically reconstructing the information, offers new and faster ways to make the most of a hologram. We describe a new method to determine the rough size of particles in an in-line hologram. This method relies on a property that is specific to interference patterns in Fresnel holograms: Self-correlation of a hologram provides access to size information. The proposed method is both simple and fast and gives results with acceptable precision. It suppresses all the problems related to the numerical depth of focus when large depth volumes are analyzed.

