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Updated: Aug 11, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Published on: October 2, 2017
Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling
Gerald V Denis1, Mark E McComb, Douglas V Faller
1Pharmacology and Medicine, Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA. gdenis@bu.edu
Abstract:
We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.
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