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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Proteome of the bacterium Mycoplasma penetrans
Mario Ferrer-Navarro1, Antonio Gómez, Oscar Yanes
1Institut de Biotecnología i de Biomedicina and Departament de Bioquímica i Biología Molecular, Universitat Autonoma de Barcelona. 08193, Bellaterra, Barcelona, Spain.
Abstract:
A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range.
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