Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective decrease of SN1(SNAT3) mRNA expression in human and rat glioma cells adapted to grow in acidic medium.

Marta Sidoryk1, Marta Obara, Jan Albrecht

  • 1Department of Neurotoxicology, Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Pawińskiego St. 5, Poland.

Neurochemistry International
|March 4, 2006
PubMed
Summary

System N glutamine transporter SN1(SNAT3) is overexpressed in malignant gliomas. However, this study found that acidic conditions decrease SN1(SNAT3) mRNA expression in glioma cells, suggesting it is not involved in adapting to the acidic tumor microenvironment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aberrant Potassium Handling by Astrocytes and Epileptic Seizures: A Synthetic Update.

Acta physiologica (Oxford, England)·2026
Same author

Oedematic-atrophic astrocytes in hepatic encephalopathy.

Acta neuropathologica communications·2025
Same author

Neuroglia in hepatic encephalopathy.

Handbook of clinical neurology·2025
Same author

Methionine Sulfoximine as a Tool for Studying Temporal Lobe Epilepsy: Initiator, Developer, Attenuator.

Neurochemical research·2025
Same author

Subtle signs of atrial cardiomyopathy and left ventricular diastolic dysfunction are associated with reduced cognitive function: results from the Hamburg City Health Study.

Clinical research in cardiology : official journal of the German Cardiac Society·2024
Same author

Attenuation of initial pilocarpine-induced electrographic seizures by methionine sulfoximine pretreatment tightly correlates with the reduction of extracellular taurine in the hippocampus.

Epilepsia·2023

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Physiology

Background:

  • System N glutamine transporter SN1(SNAT3) is overexpressed in malignant glioma cells.
  • The role of SN1(SNAT3) in glioma growth and adaptation to the tumor microenvironment is unknown.
  • Acidification of the extracellular space is a common feature of glial tumors.

Purpose of the Study:

  • To investigate the role of SN1(SNAT3) and other glutamine transporters in glioma cell adaptation to acidic conditions.
  • To determine if SN1(SNAT3) expression or function is affected by extracellular acidity.

Main Methods:

  • Human (T98G) and rat (C6) glioma cells were incubated in acidic medium (pH 6.5).
  • Messenger RNA (mRNA) expression of glutamine transporters (SN1(SNAT3), ASCT2, ATA1, SN2(SNAT5)) was measured.

Related Experiment Videos

  • Cell viability was assessed using MTT assay, and intracellular pH was measured using a fluorescent probe (BCECF-AM).
  • Glutamine uptake was measured using [(3)H]Gln.

    • Main Results:

    • Glioma cells adapted to acidic conditions, maintaining a slightly alkaline intracellular pH without significant cell loss.
    • Acidic conditions significantly decreased SN1(SNAT3) mRNA expression in both T98G and C6 cells.
    • Expression of other glutamine transporters (ASCT2, ATA1, SN2(SNAT5)) remained unaffected by acidic conditions.
    • Total glutamine uptake was not affected by acidic conditions, and the system N-mediated component was only slightly decreased.
    • System ASCT2 mediated the majority of glutamine uptake in glioma cells.

    Conclusions:

    • SN1(SNAT3) is unlikely to be involved in the adaptation of cultured glioma cells to an acidic milieu.
    • The observed decrease in SN1(SNAT3) expression under acidic conditions suggests a downregulation rather than an adaptive role.
    • Further research is needed to elucidate the specific functions of SN1(SNAT3) in glioma progression.