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Related Experiment Videos

Xenopus oocyte maturation does not require new cyclin synthesis.

J Minshull1, A Murray, A Colman

  • 1Department of Physiology, University of California, San Francisco 94143-0444.

The Journal of Cell Biology
|August 1, 1991
PubMed
Summary

New cyclin synthesis is not required for Xenopus oocyte maturation. Pre-existing stores of cyclin B1 and B2 are sufficient for progression through meiosis I and entry into meiosis II, demonstrating cyclin A is not essential.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Progesterone triggers Xenopus oocytes to undergo meiosis I and arrest at meiosis II.
  • Two distinct phases of protein synthesis are necessary to activate maturation-promoting factor (MPF) for both meiotic divisions.

Purpose of the Study:

  • To investigate the necessity of new cyclin synthesis for Xenopus oocyte entry into meiosis I and II.
  • To determine the role of cyclin A in Xenopus oocyte meiosis.

Main Methods:

  • Utilized antisense oligonucleotides to degrade maternal cyclin messenger RNAs (mRNAs) in stage VI Xenopus oocytes.
  • Assessed the requirement for de novo cyclin synthesis during meiotic progression.

Main Results:

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  • New cyclin synthesis is not required for oocytes to enter meiosis I or meiosis II.
  • Existing stores of cyclin B1 and cyclin B2 are sufficient for MPF reactivation and entry into meiosis II.
  • Cyclin A is absent in stage VI oocytes and is not required for Xenopus oocyte meiosis.

    • Conclusions:

    • Meiosis progression in Xenopus oocytes relies on pre-existing cyclin B polypeptides, not newly synthesized ones.
    • Cyclin A plays no role in the meiotic process of Xenopus oocytes.
    • Further investigation is needed to identify the specific proteins synthesized that are required for inducing meiosis I and II.