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Related Experiment Videos

Split-sample analysis of discarded cells from liquid-based Pap smear sampling devices.

Allen C Rinas1, Benjamin W Mittman, Linda Van Le

  • 1Cytotechnology Program, Department of Allied Health Sciences, University of North Carolina at Chapel Hill, USA. arinas@med.unc.edu

Acta Cytologica
|March 7, 2006
PubMed
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Discarded cells from ThinPrep Pap tests can contain abnormal cervical cells, though typically fewer than the main sample. This study found residual abnormal cells on sampling devices, impacting lesion detection sensitivity.

Area of Science:

  • Gynecologic cytology
  • Cervical cancer screening

Background:

  • Liquid-based cytology (LBC) like the ThinPrep Pap test is standard for cervical cancer screening.
  • Sampling devices in LBC collect cells, with some material potentially discarded.

Purpose of the Study:

  • To evaluate the quantity and significance of cells retained on ThinPrep Pap smear sampling devices.
  • To compare residual cells on discarded devices with the primary ThinPrep slide.

Main Methods:

  • 100 ThinPrep Pap smears were prospectively collected.
  • Sampling devices were processed using SurePath preservative fluid and slide processor.
  • Slides from discarded devices (TA material) were reviewed and compared to primary ThinPrep slides.

Main Results:

Related Experiment Videos

  • 25% of TA samples had equal or greater squamous cells than primary slides; 8% had greater cellularity.
  • Endocervical component present in 40.9% of cervical samples.
  • Abnormal cells (ASCUS, LSIL, HSIL) were found on TA slides in 60%, 85.71%, and 50% of cases, respectively.
  • Miscellaneous findings included benign endometrial cells, Candida, and histiocytic giant cells.

Conclusions:

  • TA specimens are less sensitive for cervical lesion detection than primary ThinPrep specimens.
  • A significant percentage of discarded ThinPrep sampling devices contain normal and abnormal cells.
  • Discarded abnormal cells were generally few, except in one high-grade lesion case.