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Updated: Aug 11, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
Published on: August 21, 2016
Gene variants in noncoding regions and their possible consequences
Guang-Ji Wang1, Ping Yang, Hong-Guang Xie
1China Pharmaceutical University, Key Laboratory of Drug Metabolism and Pharmacokinetics, 1 Shennong Road, Nanjing, Jiangsu 210038, People's Republic of China.
Abstract:
Human biodiversity or individual traits are not well explained by exonic mutations of all 20,000 known human genes. Accumulating evidence has demonstrated that not all noncoding regions are junk DNA sequences, and that some functionally important noncoding variants contribute significantly to altered gene expression, qualitatively or quantitatively. Thus, functional profiling or clinical relevance of noncoding variations should not be underestimated or ignored. To validate these concepts, some important examples are discussed further in this short review.
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