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Related Experiment Videos

Insertion sequence IS900 revisited.

Makeda Semret1, Christine Y Turenne, Marcel A Behr

  • 1McGill University Health Center, Montreal, Quebec, Canada.

Journal of Clinical Microbiology
|March 7, 2006
PubMed
Summary
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The IS900 genetic marker in Mycobacterium avium subsp. paratuberculosis is highly conserved, with limited variation between sheep and cattle strains. Detecting IS900-like sequences alone is insufficient for identifying this bacterium in Crohn's disease research.

Area of Science:

  • Microbiology
  • Genetics
  • Gastroenterology

Background:

  • Mycobacterium avium subsp. paratuberculosis (MAP) is investigated as a potential factor in Crohn's disease.
  • Molecular detection of the IS900 insertion sequence is a common method for identifying MAP.
  • Concerns exist regarding the reliability of IS900 detection due to reported polymorphisms.

Purpose of the Study:

  • To analyze the genetic conservation of the IS900 insertion sequence in Mycobacterium avium subsp. paratuberculosis isolates.
  • To determine if IS900 polymorphisms represent distinct variants or are indicative of conserved lineages.

Main Methods:

  • Analysis of 23 Mycobacterium avium subsp. paratuberculosis isolates.
  • Molecular characterization of the IS900 insertion sequence across different isolates.

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Main Results:

  • The IS900 insertion sequence was found to be highly conserved among the analyzed MAP isolates.
  • Only two distinct sequevars of IS900 were identified, correlating with sheep and cattle host lineages.
  • Amplification of IS900-like sequences alone is not a reliable indicator for the presence of MAP.

Conclusions:

  • IS900 is a highly conserved genetic marker in Mycobacterium avium subsp. paratuberculosis.
  • The limited variability of IS900 does not support the existence of numerous distinct MAP variants based on this sequence.
  • Reliance on IS900-like sequence amplification as a sole diagnostic proxy for MAP in clinical samples, including those related to Crohn's disease, is not scientifically supported.