Related Experiment Video
Updated: Aug 11, 2026
Determination of Microbial Biomass in Soil using Digital Droplet PCR (ddPCR)
Published on: April 24, 2026
Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method
H Aoshima1, A Kimura, A Shibutani
1Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi, Kusatsu, Shiga, 525-8577, Japan.
Abstract:
A simple and rapid method (slow-stirring method) for extracting environmental DNA (eDNA) from soils was constructed by physical mild stirring with chemical treatment. eDNA was extracted efficiently with minimal damage from various kinds of soil. The amount of eDNA and soil bacterial biomass showed a linear proportional relation [Y=(1.70x10(8))X, r2=0.96], indicating that bacterial biomass could be evaluated by quantifying levels of eDNA. Consequently, the average bacterial biomass in an agricultural field was calculated as 5.95x10(9) cells/g sample, approximately 10-100 times higher than that in non- and oil-polluted fields.
More Related Videos
09:55Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Published on: May 2, 2018
07:24A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Published on: December 10, 2014
