[Study on sex-identification of ruminant using SRY gene and microsatellite markers]

Multiplex PCR amplification of four microsatellite markers on chromosomes X and Y and the SRY gene was used to identify the sex and genotype the ruminant simultaneously in one step. Result indicated that the d SRY primers was highly specific and could be used as the main basis for sex-identification. But primer specificity was not good for Y chromosome markers MCM158 and MAF45, and therefore these markers were not suited for sex identification. The primers of two X chromosome markers MILVET09 and AE25 are amplified, but the SRY gene is not, then the individual being examined is female. Furthermore the genotype of MILVET09 and AE25 dose not affect sex determination of the female. The genotype of markers MCM158 and MAF45 dose not affect the result of sex-identification, only were able to further confirm the male identity.

when SRY, MCM158 and MAF45 on chromosome Y and MILVET09 and AE25 on chrosomome X can all be amplified simultaneously, and when the genotype of MILVET09 and AE25 is homozygous, the animal is determined to be normal male; When only MCM158, MAF45 and MILVET09 and AE25 are amplified, but the SRY gene is not, then the individual being examined is female. Furthermore the genotype of MILVET09 and AE25 does not affect sex determination of the female. The genotype of markers MCM158 and MAF45 dose not affect the result of sex-identification.