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[Study on mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP]
Xiang-Long Li1, Zeng-Li Zhang, Yuan-Fang Gong
1Department of Animal Science and Technology, Hebei Agriculture University, Baoding 071001, China. lixianglongcn@yahoo.com
Abstract:
The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polymorphic degree (Pi value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.
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