Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A universal high-throughput novel method of constructing the vectors].

Yun-Hua Lu1, Li-Xin Ma, Si-Jing Jiang

  • 1Laboratory of Molecular Microbiology and Gene Engineering, College of Life Science, Hubei University, Wuhan 430062, China. lyh655@yahoo.com.cn

Yi Chuan = Hereditas
|March 8, 2006
PubMed
Summary

This study introduces a novel, high-throughput method for constructing complex DNA vectors. This simple technique streamlines vector construction, significantly reducing experimental steps for applications like chloroplast expression vectors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional analysis of nuclear receptor genes in molting and metamorphosis of the cigarette beetle, Lasioderma serricorne.

International journal of biological macromolecules·2024
Same author

Lycium barbarum polysaccharide-glycoprotein ameliorates ionizing radiation-induced epithelial injury by regulating oxidative stress and ferroptosis via the Nrf2 pathway.

Free radical biology & medicine·2023
Same author

Understanding the inter-city causality and regional transport of atmospheric PM<sub>2.5</sub> pollution in winter in the Harbin-Changchun megalopolis in China: A perspective from local and regional.

Environmental research·2023
Same author

Phosphorylation of covalent organic framework nanospheres for inhibition of amyloid-β peptide fibrillation.

Chemical science·2022
Same author

Ursolic Acid Protects Neurons in Temporal Lobe Epilepsy and Cognitive Impairment by Repressing Inflammation and Oxidation.

Frontiers in pharmacology·2022
Same author

Antibacterial and antibiofilm activities of novel antimicrobial peptide DP7 against the periodontal pathogen Porphyromonas gingivalis.

Journal of applied microbiology·2022

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetic Engineering

Context:

  • Traditional vector construction can be complex and time-consuming.
  • High-throughput methods are crucial for accelerating biological research.
  • Efficient gene cloning and vector assembly are fundamental in molecular biology.

Purpose:

  • To develop a simple, universal, and high-throughput method for constructing complex DNA vectors.
  • To streamline the process of assembling multiple DNA fragments into a vector.
  • To enable efficient and oriented recombination of DNA fragments.

Summary:

  • The method utilizes PCR primers with adapters and T4 DNA polymerase for creating complementary sticky ends on DNA fragments.
  • Fragments are then assembled into vectors using DNA ligase, facilitating oriented recombination.

Related Experiment Videos

  • Demonstrated with the construction of the Oryza sativa chloroplast expression vector pRSMGA, reducing steps to two recombination and transformation cycles.

    • Impact:

    • Significantly simplifies and accelerates the construction of complex vectors.
    • Offers a universal and high-throughput approach applicable to various vector construction needs.
    • Potential to advance research in plant biotechnology and other fields requiring complex vector assembly.