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Updated: Jul 20, 2026

Generation of Human CD40-activated B cells
Published on: October 16, 2009
Role of CD80 in stimulating T lymphocyte activation
De-Chun Li1, Xing-Guo Zhu, Yong Zhang
1Department of General Surgery, the First Affiliated Hospital of Soochow University, 96 Shizi Road, Suzhou 215006, Jiangsu Province, China.
Aim:
To observe biological characteristics of hepatocarcinoma cells before and after CD80 transfection and to compare the effect of CD80-transfected hepatocarcinoma cells on T lymphocyte activation.
Methods:
Retro virus vector carrying CD80 gene was transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells (HepG2/hCD80). Flow cytometry (FCM) was performed to detect CD80 expression in the transfected cells. RT-PCR was used to evaluate CD80 expression at mRNA level. In the presence of anti-CD3 mAb, the proliferation of T lymphocyte was observed by MTT. Meanwhile, the expression of activated molecule marker CD25 was analyzed through FCM.
Results:
A stable cell line HepG2/hCD80 expressing the human CD80 was established. Growth curve showed that the molecule CD80 could obviously decrease the growth of tumor cells. HepG2/hCD80 was evidenced to have a potency to enhance T cell proliferation and upregulate CD25 expression.
Conclusion:
CD80 transfection can lower malignant phenotype of hepatocarcinoma cells. CD80 transfection has a down-regulatory effect to activated T cells in vitro.
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