[Chromatographic methods in tobacco smoke assessment]

In this paper review of chromatographic techniques and detectors used to analyse nicotine and its metabolites has been presented. The chromatographic determination of xenobiotics in biological material is usually proceeded by multistage isolation procedures, using liquid-liquid and liquid-solid extraction in various modifications. In our investigations, planar chromatography with densitometry has been used to identify and quantify nicotine and its metabolites in body fluids for assessment of tobacco smoke exposure. This technique does not require special cleanup techniques and as well is economic and environmental friendly.